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产品性质
biological source【生物来源】 | human blood |
growth mode【生长模式】 | Suspension |
karyotype【核型】 | Modal no. = 93, Hypertetraploid |
morphology【形态学】 | Lymphoblastoid |
products【产品】 | Not specified |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
relevant disease(s)【相关疾病】 | cancer |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Human Caucasian promyelocytic leukaemia |
Cell Line Description【细胞系描述】 | Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Cells are capable of differentiating into neutrophils by induction with 1.75% DMSO. |
Application【应用】 | Differentiation studies |
DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X CSF1PO: 14 D13S317: 8,11 D16S539: 11 D5S818: 12 D7S820: 11,12 THO1: 7 TPOX: 8,11 vWA: 16 |
Culture Medium【培养基】 | RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO). |
Subculture Routine【传代培养常规】 | Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37℃. Do not allow the culture medium to become acidic or the cell line will differentiate. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |