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产品性质
biological source【生物来源】 | human blood |
growth mode【生长模式】 | Suspension |
karyotype【核型】 | Not specified |
morphology【形态学】 | Not specified |
products【产品】 | Not specified |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
relevant disease(s)【相关疾病】 | cancer |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Human Caucasian promyelocytic leukaemia |
Cell Line Description【细胞系描述】 | HL60 15-12 is a variant of HL60 which is capable of differentiating towards either neutrophils or monocytes with treatment of appropriate agents. |
Culture Medium【培养基】 | RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO). |
Subculture Routine【传代培养常规】 | Maintain cultures between 2x100,000 to 1,000,000 cells/ml. NB: The cells will differentiate if the culture medium is allowed to become acidic. 5% CO2; 37℃. Freeze in 10% glycerol and 90% FBS. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |