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Histone Deacetylase 8 (HDAC8) Activity Assay Kit

产品编号:3097055
规格:100 assays in 96 well plates
包装规格:1 KIT
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
30970551 KIT6590
基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
3.5.1.98 ( BRENDA | IUBMB )
NACRES
NA.41
General description【一般描述】
Histone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, it plays a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence.

With Sigma′s HDAC8 Activity Assay Kit, HDAC8 present in a test sample will act with the supplied Developer, to deacetylate and then cleave the HDAC8 Substrate (R-H-K(Ac)-K(Ac)-AFC). This activity will release the quenched fluorescent group, AFC, which can be detected at Em/Ex = 380/500 nm. Trichostatin A is an HDAC inhibitor included in the kit to verify HDAC8 activity. The kit provides a rapid, simple, sensitive, and reliable test. It is suitable for either individual tests or high throughput assays, from nuclear extracts, purified, or immunoprecipitated HDAC8, and from native, recombinant, or genetically modified HDAC8.
HDAC8 (histone deacetylase 8) is a class I enzyme, that has 377 residues and it lies between class I and class II HDACs. This gene is located on X chromosome. It consists of a single α/β domain, in which eight parallel stranded β sheets are sandwiched between 13 α helices.
Biochem/physiol Actions【生化/生理作用】
HDAC8 (histone deacetylase 8), with the help of PKA (cyclic AMP-dependent protein kinase A) plays a major role in confirming the acetylation state of histones. This protein may also plays an important role in acute myeloid leukemia (AML). Suppression of HDAC8 by RNA interference stops the growth of lung, colon, and cervical cancer cell lines.
Features and Benefits【特点和优势】
  • Simple, sensitive, and reliable assay
  • Utilizes fluorometric methods
  • Sample type: cell and tissue lysates, plasma and serum, other biological fluids
  • Species reactivity: mammalian
  • Suitable for individual tests or high throughput assays and kinetic studies
  • Suitable for high throughput measurement of HDAC8 activity in purified, immunoprecipitated, and recombinant or genetically modified HDAC8 samples
  • Convenient 96-well microplate format
产品性质
Quality Level【质量水平】
100
usage【用途】
100 assays in 96 well plates
NCBI accession no.【NCBI登记号】
NM_001166418 (Human)
NM_027382 (Mouse)
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
Gene Information
human ... HDAC8(55869)
mouse ... HDAC8(70315)
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
Flash Point(F)【闪点(F)】
188.6 °F - closed cup
Flash Point(C)【闪点(C)】
87 ℃ - closed cup
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