您的位置:首页 > 产品中心 > Histone Deacetylase 8 (HDAC8) Activity Assay Kit
Histone Deacetylase 8 (HDAC8) Activity Assay Kit
基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.5.1.98 ( BRENDA | IUBMB ) |
NACRES | NA.41 |
General description【一般描述】 | Histone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, it plays a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. With Sigma′s HDAC8 Activity Assay Kit, HDAC8 present in a test sample will act with the supplied Developer, to deacetylate and then cleave the HDAC8 Substrate (R-H-K(Ac)-K(Ac)-AFC). This activity will release the quenched fluorescent group, AFC, which can be detected at Em/Ex = 380/500 nm. Trichostatin A is an HDAC inhibitor included in the kit to verify HDAC8 activity. The kit provides a rapid, simple, sensitive, and reliable test. It is suitable for either individual tests or high throughput assays, from nuclear extracts, purified, or immunoprecipitated HDAC8, and from native, recombinant, or genetically modified HDAC8. HDAC8 (histone deacetylase 8) is a class I enzyme, that has 377 residues and it lies between class I and class II HDACs. This gene is located on X chromosome. It consists of a single α/β domain, in which eight parallel stranded β sheets are sandwiched between 13 α helices. |
Biochem/physiol Actions【生化/生理作用】 | HDAC8 (histone deacetylase 8), with the help of PKA (cyclic AMP-dependent protein kinase A) plays a major role in confirming the acetylation state of histones. This protein may also plays an important role in acute myeloid leukemia (AML). Suppression of HDAC8 by RNA interference stops the growth of lung, colon, and cervical cancer cell lines. |
Features and Benefits【特点和优势】 |
|
产品性质
Quality Level【质量水平】 | 100 |
usage【用途】 | 100 assays in 96 well plates |
NCBI accession no.【NCBI登记号】 | NM_001166418 (Human) NM_027382 (Mouse) |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... HDAC8(55869) mouse ... HDAC8(70315) |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
Flash Point(F)【闪点(F)】 | 188.6 °F - closed cup |
Flash Point(C)【闪点(C)】 | 87 ℃ - closed cup |