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产品性质
| biological source【生物来源】 | human cervix |
| growth mode【生长模式】 | Suspension |
| karyotype【核型】 | Modal no. 68 |
| morphology【形态学】 | Epithelial |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human cervix carcinoma |
| Cell Line Description【细胞系描述】 | Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line. Very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition and plaque forming ability. The cells should be handled under laboratory containment level 2. |
| Application【应用】 | HeLa S3 has been used to study the cytotoxicity of dinuclear titanium-salan complexes. It has also been used to perform in vitro genotoxicity tests (comet and micronucleus) to study the effects of occupational exposure to carcinogens on sewage workers. |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X CSF1PO: 9,10 D13S317: 14 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Culture Medium【培养基】 | SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS) |
| Subculture Routine【传代培养常规】 | Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37℃. Culture in a spinner flask. This cell line may adhere if the culture is not agitated. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
