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HeLa DH

产品编号:3095521
规格:96112022
包装规格:5μg,1 VIAL
产品类别:进口试剂
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
30955215μg4630
30955211 VIAL11270
产品性质
biological source【生物来源】
human cervix
growth mode【生长模式】
Adherent
karyotype【核型】
Not specified
morphology【形态学】
Epithelial
products【产品】
Not specified
receptors【受体】
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)【相关疾病】
cancer
shipped in【运输】
dry ice
storage temp.【储存温度】
−196℃
基本信息
Cell Line Origin【细胞系来源】
Human cervix carcinoma
Cell Line Description【细胞系描述】
HeLa DH cells are the wildtype that has been used for connexin-transfection assays and subsequent analysis of permeability and gap junction formation. The wildtype exhibits stable membrane potentials, very low gap junctional conductance and high stability under double whole-cell patch-clamp measurements. Gap junction plaques were not detected but using antibody directed against connexin 45 identified gap junction channels as dispersed particles in non typical gap junction areas.
Application【应用】
Transfection assays.
Culture Medium【培养基】
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine【传代培养常规】
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cell/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
Other Notes【其他说明】
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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