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H2.35

产品编号:3094419
规格:94050407
包装规格:5μg,1 VIAL
产品类别:进口试剂
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
30944195μg4630
30944195μg5400
30944191 VIAL9720
产品性质
biological source【生物来源】
mouse liver
growth mode【生长模式】
Adherent
karyotype【核型】
Not specified
morphology【形态学】
Epithelial
products【产品】
Not specified
receptors【受体】
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in【运输】
dry ice
storage temp.【储存温度】
−196℃
基本信息
Cell Line Origin【细胞系来源】
Mouse Hepatocyte, SV40 (tsA255) transformed
Cell Line Description【细胞系描述】
From a primary hepatocyte culture of 6 week old female BALB/c mouse transformed with tsA255 (temperature-sensitive mutant of SV40 virus). H2.35 is temperature sensitive for expression of serum albumin mRNA. During routine propagation at 33℃ it exhibits extremely low levels of albumin transcription and mRNA, while at the restructure temp of 39℃ albumin mRNA increases 100 fold if the cells are grown in serum free medium on a collagen substratum. Undergoes morphological changes in response to extracellular matrix gel substrata, albumin gene transcription is induced. Liver-enriched transcription factors are induced in serum-free medium and on collagen gels. The cells should be handled under laboratory containment level 2.
Application【应用】
Provides modal system for studying hepatocyte differentiation because some hepatocyte phenotypes are inducible in the cells. Transfection recipient for assays of genetic regulatory sequence function for liver genes that do not function in other cells.
Culture Medium【培养基】
DMEM (containing 1g / l glucose) + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 4% Foetal Bovine Serum (FBS).
Subculture Routine【传代培养常规】
Split sub-confluent cultures (70-80%) 1:6 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 33℃.
Other Notes【其他说明】
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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