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糖肽酶 A 来源于杏仁

Glycopeptidase A from almonds
产品编号:3091730
规格:buffered aqueous glycerol solution, ≥0.05 unit/mL
CAS NO:83534-39-8
包装规格:0.005 UNIT
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
30917300.005 UNIT10890
产品别名

83534-39-8

糖肽酶 A 来源于杏仁

Glycopeptidase A from almonds

N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase

N-Glycosidase A

PNGase A

基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
3.5.1.52 ( BRENDA | IUBMB )
MDL number
MFCD00131222
NACRES
NA.32
General description【一般描述】
Glycopeptidase found in almonds can be divided into three groups- A, B and C. the optimum pH value and the isoelectric point of glycopeptidase A is 6.0 and 7.7 respectively. It has a preference for glycopeptides with long chains. It is also capable of hydrolyzing intact glycoproteins such as, desialyted human transferrin, ovalbumin etc. These proteins cleave glycoproteins with asialocarbohydrate moieties at their β-aspartyl-glucosylamine linkages.
Application【应用】
Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp.
Biochem/physiol Actions【生化/生理作用】
Hydrolyzes an N4-(acetyl-β-D-glycosaminyl)asparagine in which the N-acetyl-D-glucosamine residue may be further glycosylated, yielding a (substituted) N-acetyl-β-D-glucoaminylamine and the peptide containing an aspartic residue.
Unit Definition【单位定义】
One unit will hydrolyze 1.0 μmole of ovalbumin glycopeptide per min at pH 5.0 at 37℃.
Physical form【外形】
Solution in 50% glycerol containing 50 mM citrate-phosphate buffer, pH 5.0, and BSA.
产品性质
form【形式】
buffered aqueous glycerol solution
Quality Level【质量水平】
200
concentration【浓度】
≥0.05 unit/mL
storage temp.【储存温度】
−20℃
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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