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增强型禽类第一链合成试剂盒
Enhanced Avian First Strand Synthesis Kit
产品编号: | 3080997 |
规格: | Components for cDNA synthesis with enhanced AMV reverse transcriptase |
包装规格: | 1 KIT |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
增强型禽类第一链合成试剂盒
Enhanced Avian First Strand Synthesis Kit
基本信息
NACRES | NA.55 |
Application【应用】 | 增强的 Avian第一链合成试剂盒,应用了一款高度纯化的禽成髓细胞瘤病毒逆转录酶(eAMV-RT),后者的反应性能远超标准型AMV-RT。这一高效eAMV-RT在高温(高达65 ℃)能够下可打开RNA的复杂二级结构,是以总RNA或poly(A)+ RNA为模板 制备高质量全长cDNA的理想选择。 |
Unit Definition【单位定义】 | 一单位酶可以多聚腺苷酸为模板,以12-18长度的oligo(dT)为引物,在10分钟内将1纳摩TMP整合进TCA-沉淀材料中。 |
Legal Information【法律信息】 | Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. |
Components【组分】 | 组份不可单独销售 Enhanced Avian Reverse Transcriptase 1000 U |
产品性质
Quality Level【质量水平】 | 200 |
usage【用途】 | sufficient for 50 reactions kit sufficient for 50 reactions |
feature【特点】 | dNTPs included hotstart: no |
technique(s) | RT-PCR: suitable |
color【颜色】 | colorless |
input | purified RNA |
web metadata keyword.default | cDNA; PCR; RT; DNA synthesis kit; STR1; RT-PCR; AMV-RT; eAMV-RT; MMLV-RT; first strand; high temperature; reverse transcriptase polymerase chain reaction |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 3 |