广州伟伯科技有限公司
您的位置:首页 > 产品中心 > DI TNC1
产品搜索:

DI TNC1

产品编号:3075321
规格:98102214
包装规格:5μg,1 VIAL
产品类别:进口试剂
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
30753215μg4630
30753211 VIAL9720
产品性质
biological source【生物来源】
rat brain
growth mode【生长模式】
Adherent
karyotype【核型】
Not specified
morphology【形态学】
Neuronal (astrocyte)
products【产品】
Alpha-2 macroglobulin
receptors【受体】
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in【运输】
dry ice
storage temp.【储存温度】
−196℃
基本信息
Cell Line Origin【细胞系来源】
Rat Astrocyte transfected
Cell Line Description【细胞系描述】
Established by transfecting cultures of primary astrocytes from diencephalon tissue of a 1 day old sprague-dawley rat with a DNA construct containing the oncogenic early region of SV40. Transcriptional control was affected using the human GFAP promoter (pGFA-SV-Tt) and the murine phosphoglycerate kinase promoter (pPGK-neo). Cloning was achieved using G418. The cells are phenotypically similar to type 1 astrocytes, including immunoreactivity to glial fibrillary acid protein (GFAP) and a β-alanine inhibitable high-affinity uptake mechanism for gamma amino butyric acid (GABA). α-2-macroglobulin production is similar to that found in primary astrocytes, but transferrin production is reduced. The cell line has been shown not to produce proenkephalin A, galactocerebroside or to express the 04 or A2B5 epitopes characteristic of type 2 astrocytes. Immunostaining has shown the SV40 T antigen to be present in over 90% of cells.
Application【应用】
Studies of the development and biochemistry of the central neurosystem
Culture Medium【培养基】
DMEM + 4mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculture Routine【传代培养常规】
Split sub-confluent cultures (70-80%) at 1:2 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. Subculture before flasks become confluent or cells will detach in sheets reducing effectiveness of trypsin.
Other Notes【其他说明】
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
广州伟伯科技有限公司 版权所有 CopyRight ©2006-2024, All Rights Reserved
工信部备案号:粤ICP备08114744号   Page Run Time: 0.0076