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Deoxyribonuclease II from porcine spleen
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产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
3074992 | 20000 UNITS | 763 | |||
3074992 | 80000 UNITS | 2240 |
产品别名
9025-64-3
Deoxyribonuclease II from porcine spleen
Deoxyribonucleate 3′-oligonucleotido-hydrolase
DNase II
基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.1.22.1 ( BRENDA | IUBMB ) |
EC number【EC 号】 | 232-801-8 |
MDL number | MFCD00130919 |
NACRES | NA.54 |
Application【应用】 | Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane. DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). |
Biochem/physiol Actions【生化/生理作用】 | The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass. |
Unit Definition【单位定义】 | One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25℃; [Mg2+] = 0.83 mM |
Physical form【外形】 | 含氯化钠 |
产品性质
Quality Level【质量水平】 | 200 |
type【类型】 | Type IV |
form【形式】 | lyophilized powder |
Specific Activity | 2,000-6,000 Kunitz units/mg protein (biuret) |
mol wt【分子量】 | 45 kDa |
composition【组成】 | Protein, 60-80% |
foreign activity【异质活性】 | RNase ≤0.05% |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 20000, 80000 units in poly bottle |
安全信息
Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
WGK | WGK 3 |