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Deoxyribonuclease II from porcine spleen

产品编号:3074992
规格:Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)
CAS NO:9025-64-3
包装规格:20000 UNITS,80000 UNITS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
307499220000 UNITS763
307499280000 UNITS2240
产品别名

9025-64-3

Deoxyribonuclease II from porcine spleen

Deoxyribonucleate 3′-oligonucleotido-hydrolase

DNase II

基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
3.1.22.1 ( BRENDA | IUBMB )
EC number【EC 号】
232-801-8
MDL number
MFCD00130919
NACRES
NA.54
Application【应用】
Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.
DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).
Biochem/physiol Actions【生化/生理作用】
The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.
Unit Definition【单位定义】
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25℃; [Mg2+] = 0.83 mM
Physical form【外形】
含氯化钠
产品性质
Quality Level【质量水平】
200
type【类型】
Type IV
form【形式】
lyophilized powder
Specific Activity
2,000-6,000 Kunitz units/mg protein (biuret)
mol wt【分子量】
45 kDa
composition【组成】
Protein, 60-80%
foreign activity【异质活性】
RNase ≤0.05%
storage temp.【储存温度】
−20℃
packaging【包装】
20000, 80000 units in poly bottle
安全信息
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 3
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