MRP1 Knockout Caco-2 Cells

NA.81

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP1 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC1 (MRP1) efflux transporter.

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:

• A functional knockout of the MRP1 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP1 substrate
• The vial format enables the MRP1 knockout cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

ADME/Tox Cell Lines License

200

human male colorectal tissue (Source Disease: colon adenocarcinoma)

liquid

drug transporter assay: suitable
permeability assay: suitable

ADME/TOX

−196℃

human ... ABCC1(4363)

12 - Non Combustible Liquids

WGK 3