Anti-DNA-PK (Ab-2) Mouse mAb (18-2)

100

mouse

purified antibody

primary antibodies

18-2, monoclonal

liquid

≤0.1% sodium azide as preservative

human

Calbiochem^®

OK to freeze

IgG1

wet ice

2-8℃

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

This Anti-DNA-PK (Ab-2) Mouse mAb (18-2) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of DNA-PK (Ab-2).
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing the splenocytes with mouse myeloma SP2/0 cells (see application references). Recognizes the ~460 kDa DNA-PK protein.
Recognizes the ~470 kDa DNA-PK protein in A431, HeLa, and HL60 cells, normal tonsil, normal colon, and breast carcinoma tissue.

DNA-PK purified from HeLa cells
Epitope: within amino acids 1-2713 of DNA-PK
Human

Frozen Sections (1:50-1:100)
Immunoblotting (1:250-1:500)
Immunofluorescence (1:50-1:100)
Immunoprecipitation (10 l/mg protein lysate)
Neutralization Studies (see comments)
Paraffin Sections (1:50-1:100, heat/pressure cooker pre-treatment required)

Toxicity: Standard Handling (A)

In 10 mM PBS, 0.2% BSA, pH 7.4.

For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

Positive Control
A-431, HeLa, or HL-60 cells or normal tonsil, normal colon, or breast carcinoma tissue

Kharbanda, S., et al. 1997. Nature386, 732.
McConnell, K.R., et al. 1997 J. Immunol.158, 2083-2089.
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Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol.217, 91.
Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10285.
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Connelly, M.A., et al. 1996. Gene175, 271.
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Lees-Miller, S.P. 1996. Biochem. Cell. Biol.74, 503.
Song, Q., et al. 1996. EMBO J.15, 3238.
Teraoka, H., et al. 1996. FEBS Lett.393, 1.
Blunt, T., et al. 1995. Cell80, 813.
Hartley, K.O., et al. 1995. Cell82, 849.
Kirchgessner, C.U., et al. 1995. Science267, 1178.
Lees-Miller, S.P., et al. 1995. Science267, 1183.
Poltoratsky, V.P., et al. 1995. J. Immunol.155, 4529.
Anderson, C.W. 1993. Trends Biochem. Sci.18, 433.
Gottleib, T.M. and Jackson, S.P. 1993. Cell72, 131.
This antibody will inhibit (approximately 50%) the kinase activity of DNA-PKcs. However, preincubation of the enzyme with DNA protects DNA-PKcs against inactivation by Clone 18-2. Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

12 - Non Combustible Liquids

WGK 2