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Anti-AFP Mouse mAb (1G7)
产品别名
Anti-AFP Mouse mAb (1G7)
Anti-α-Fetoprotein
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 1G7, monoclonal |
form【形式】 | liquid |
does not contain【不包含】 | preservative |
species reactivity | human |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
isotype【同位素/亚型】 | IgG |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 100 μg in Glass bottle |
基本信息
General description【一般描述】 | Recognizes the ~70-75 kDa AFP protein in HepG2 cells. Purified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein. Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry. |
Immunogen【免疫原】 | A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein |
Application【应用】 | ELISA (see coments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) |
Warning【警告】 | Toxicity: Regulatory Review (Z) |
Physical form【外形】 | In PBS, pH 7.2. |
Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). |
Analysis Note【分析说明】 | Positive Control HepG2 cells Negative Control 293T cells |
Other Notes【其他说明】 | For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | nwg |