Anti-Melanopsin Antibody, clone 1F8.1

32160702

Melanopsin (UniProt Q9UHM6; also known as Opsin-4) is encoded by the OPN4 (also known as MOP) gene (Gene ID 94233) in human. Opsins belong to the superfamily of G protein-coupled receptors (GPCRs). Melanopsin is a blue light-sensitive opsin protein that is capable of rendering cells intrinsically light responsive. Melanopsin expression defines a population of retinal ganglion cells that are intrinsically photosensitive (ipRGCs). Melanopsin allows mammals to measure irradiance for centrally mediated responses such as circadian entrainment. In addition, the information generated by melanopsin is used by the visual system as a signal for light adaptation. Using melanopsin-dependent phototransduction mechanism coupled with extrinsic signals originating with rods and cones, ipRGCs can encode light levels over many decimal orders, allowing the visual system to adapt to a broad range of light intensity from starlight to broad daylight.

Clone 1F8.1 is expected to react with both spliced isoforms of human melanopsin.

GST-tagged recombinant protein corresponding to human Melanopsin C-terminal sequence

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Melanopsin in human retina tissue.
This Anti-Melanopsin Antibody, clone 1F8.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Melanopsin .

Evaluated by Western Blotting in human retinal pigment epithelia (RPE) ARPE-19 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Melanopsin in 10 µg of human retinal pigment epithelia (RPE) ARPE-19 cell lysate.

Purified mouse monoclonal IgG2aλ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

1F8.1, monoclonal

human

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG2aλ

NP_150598

Q9UHM6

wet ice

~52 kDa observed

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12 - Non Combustible Liquids

WGK 1