Anti-Filamin A Antibody, clone TI10

32160702

NA.41

Filamin is a structural protein that forms flexible cross-links between two actin filaments. Filamin is a homodimer of polypeptide chains each joined to the other at one end with an actin binding site ath the other. It is present in smooth muscle, fibroblasts, platelets and lymphocytes.

Human filamin (actin-bidning protein). Recognizes unprocessed (270-280 kDa) and the C-terminal 90 kDa calpain cleavage fragment of filamin (Aakhus, 1992).

Human platelet protein

Research Category
Cell Structure
Detect Filamin A using this Anti-Filamin A Antibody, clone TI10 validated for use in IP, WB, IH, IH(P).
Research Sub Category
Cytoskeleton
Immunoblotting: 1:250 to 1:1000

Immunoprecipitation:Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5 microliters of antibody for every 300μL of cell lysate (3-5mg/ml total protein is suggested). Incubation is 4 hours RT or overnight 4C; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-10% acrylamide gels are recommended for full length filamin or the 80kDa fragement visualization.

Immunofluorescence: 1:50 to 1:200 using standard ABC technique. Suitable for staining of paraffin embedded sections (lower dilutions). High temperature citrate buffer antigen retrieval technique recommended.

Optimal working dilutions must be determined by end user.

Replaces: CBL229

Ascites. Liquid

Control
Positive control tisse: skin.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

ascites fluid

primary antibodies

TI10, monoclonal

bovine, human

mouse, rat

Chemicon^®

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1

NM_001110556.1
NM_001456.3

P21333

dry ice

Maintain frozen at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze/thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1