Anti-Cytomegalovirus Antibody, late Antigen, clone 1G5.2

32160702

Reacts with a late protein. Can detect CMV infection 24 hours post-infection exhibiting a cytoplasmic staining which reaches peak intensity at >72 hours.

With CMV the antigens expressed at different times are listed as:



Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD

Sucrose gradient purified CMV AD169 (ATCC).
Epitope: late antigen

Immunochemistry.

IFA at 1:400-1:800 on acetone fixed cells.

Works on paraffin embedded tissue sections.

Dilute with buffer pH 7.4-7.6 to desired working volume.

For extensive dilution, protein containing or other stabilizing medium should be used.

Final working dilutions must be determined by end user.
Research Category
Infectious Diseases
Detect Cytomegalovirus using this Anti-Cytomegalovirus Antibody, late antigen, clone 1G5.2 validated for use in FC, IF, IH(P).
Research Sub Category
Infectious Diseases - Viral

Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Format: Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

300

mouse

purified immunoglobulin

primary antibodies

1G5.2, monoclonal

human

Chemicon^®

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)

IgG2a

not suitable for Western blot

wet ice

Maintain at +4°C for up to 12 months

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2