Anti-CHIP Rabbit pAb

rabbit

100

serum

primary antibodies

polyclonal

liquid

≤0.1% Sodium azide as preservative

mouse, human, Drosophila, rat, hamster, monkey

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG

wet ice

−20℃

100 μL in Plastic ampoule

This Anti-CHIP Rabbit pAb is validated for use in Immunoblotting for the detection of CHIP.
Recognizes the ~35 kDa CHIP protein. May also detect bands at ~43, ~51, and ~70 kDa corresponding to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~35 kDa CHIP protein.

a recombinant protein consisting of full-length human CHIP fused to a His•Tag sequence

Immunoblotting (1:200-1:1000)

Immunofluorescence (1:250)

Immunoprecipitation (1:20)

Toxicity: Standard Handling (A)

Undiluted serum.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
COS-7 cells

May also detect bands at 43, 51, and 70 kDa, wich correspond to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. Antibody should be titrated for optimal results in individual systems.
Xu, W., et al. 2002. Proc. Natl. Acad. Sci. USA99, 12847.
Demand, J., et al. 2001. Curr. Biol.11, 1569.
Connell, P., et al. 2001. Nat. Cell Biol.3, 93.
Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
Meacham, G.C., et al. 2001. Nat. Cell. Biol.3, 100.
Ballinger, C.A., et al. 1999. Mol. Cell. Biol.19, 4535.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 1