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Rho Assay Reagent (Rhotekin RBD, agarose), 650 μg
| 产品编号： | 4101771 |
| 规格： | Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays. |
| 包装规格： | 650 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| eCl@ss | 32160405 |
| NACRES | NA.84 |
| General description【一般描述】 | GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates. Product Source: Expressed in E. coli |
| Quality【质量】 | Routinely evaluated by precipitating GTP-Rho from 3T3/A31 lysates, subsequent detection by immunoblot analysis using Anti-RhoA, clone 55 (05-778). |
| Physical form【外形】 | 650 µg of recombinant protein in 1 mL glutathione-agarose slurry containing 45mM TRIS, pH 7.5, 0.45% Triton X-100, 135mM NaCl, 4.5mM MgCl2, 0.9mM dithiothreitol, 0.09mM PMSF, 0.9 µg/mL aprotinin, 0.9 µg/mL leupeptin, and 10% glycerol. |
| Other Notes【其他说明】 | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| manufacturer/tradename | Upstate® |
| technique(s) | affinity binding assay: suitable |
| NCBI accession no.【NCBI登记号】 | NM_001015055.1 NM_001015056.1 NM_033046.2 |
| UniProt accession no.【UniProt登记号】 | Q9BST9 |
| shipped in【运输】 | dry ice |
| Gene Information | mouse ... RTKN(20166) |
产品说明
| Storage and Stability【储存及稳定性】 | Stable for 6 months at -70°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Hazard Statements | H412 |
| Precautionary Statements | P273 - P501 |
| Hazard Classifications【危险分类】 | Aquatic Chronic 3 |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
