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Anti-trimethyl Histone H3 (Lys27) Antibody
| 产品编号： | 4101172 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-trimethyl Histone H3 (Lys27) Antibody
H3K27me3, Histone H3 (tri methyl K27), H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3K27me3
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 lysine 27 methylation (H3K27) is associated with heterochromatin formation and transcriptional repression. |
| Specificity【特异性】 | Broad species cross-reactivity expected. This antibody recognizes Histone H3 methylated at Lys27. |
| Immunogen【免疫原】 | Branch peptide corresponding to human Histone H3 methylated at Lys27. Epitope: Lys27 |
| Application【应用】 | Research Sub Category Histones Research Category Epigenetics & Nuclear Function Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in NIH/3T3 cells. Peptide Inhibition Assay: This antibody peptide blocked on HeLa cell extracts. Anti-trimethyl Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody for detection of Histone H3 trimethylated on lysine 27( H3K27me3). This purified polyclonal is specificity verified by dot blot (DB), published in peer reviewed journals and validated in WB, ICC, PIA. |
| Quality【质量】 | Evaluated by Western Blot in HeLa acid extract and recombinant Histone H3. Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H3 in 10 µg of HeLa acid extract . |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control HeLa acid extract and recombinant Histone H3 |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | mouse, human |
| technique(s) | dot blot: suitable immunocytochemistry: suitable inhibition assay: suitable (peptide) western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_003484.1 |
| UniProt accession no.【UniProt登记号】 | Q16695 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... HIST1H3F(8968) |
产品说明
| Target description【目标描述】 | ~ 17 kDa |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
