Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate

32160702

NA.41

Protein tyrosine phosphorylation was first described in 1979. Large numbers of tyrosine kinases have been described since, and many of the signaling pathways initiated by tyrosine phosphorylation have been delineated. Availability of a top-quality anti-phosphotyrosine antibody has allowed the study of tyrosine kinase activity by simple blotting procedures, and without routine need of large quantities of radioactivity. The 4G10 monoclonal anti-phosphotyrosine antibody (Cat. # 05-321) has been widely recognized as a highly specific and sensitive. This antibody is suitable for all applications involving protein tyrosine phosphorylation. The slightly less sensitive, but still highly regarded clone PY20 can be used as an alternative to the 4G10 product line.

Recognizes proteins containing phosphotyrosine residues, regardless of origin.

Phosphotyrosine conjugated to carrier protein
Epitope: phosphotyrosine

Research Sub Category
General Post-translation Modification
Research Category
Signaling
Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate is a Mouse Monoclonal Antibody for the detection of Phosphotyrosine & has been validated in WB, ELISA.

Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.

Protein A purified
100 μL of protein A purified mouse IgG2b in PBS containing 0.05% Kathon^® and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:2000- 1:4000 in 10 mL blocking buffer. Liquid.

Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

peroxidase conjugate

purified immunoglobulin

primary antibodies

PY20, monoclonal

all

Upstate^®

ELISA: suitable
western blot: suitable

IgG2b

wet ice

Varies

6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS07,GHS09

Warning

H315 - H317 - H319 - H411

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

12 - Non Combustible Liquids

WGK 3