Anti-Phosphoserine Antibody, clone 4A4, HRP Conjugate

32160702

NA.41

The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotrans-mission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that over 95% of all phosphorylation events occur on serine and threonine residues.

Recognizes proteins containing phosphoserine residues, regardless of origin.

Epitope: phosphoserine
Phosphoserine conjugated to KLH.

Anti-Phosphoserine Antibody, clone 4A4, HRP Conjugate is an antibody against Phosphoserine for use in WB.
Research Sub Category
General Post-translation Modification
Research Category
Signaling

Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.

100 μL of protein G purified mouse IgG1 in PBS containing 0.05% Kathon^® and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:4000 in 10 mL blocking buffer. Liquid.
Protein G Purified

Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

peroxidase conjugate

purified antibody

primary antibodies

4A4, monoclonal

all

Upstate^®

western blot: suitable

IgG1

wet ice

Varies

6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids