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Anti-Mouse CD90.2 (30-H12) ColorWheel® Dye-Ready mAb

产品编号:4101114
规格:for use with ColorWheel® Dyes (Required, sold separately)
包装规格:25 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
410111425 μL1750
产品性质
Quality Level【质量水平】
200
biological source【生物来源】
rat
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
30-H12, monoclonal
product line【产品线】
ColorWheel®
form【形式】
lyophilized
mol wt【分子量】
calculated mol wt 18.08 kDa
species reactivity
mouse
packaging【包装】
antibody small pack of 25 μL
technique(s)
flow cytometry: suitable
isotype【同位素/亚型】
IgG2bκ
epitope sequence【表位序列】
Unknown
Protein ID accession no.【Protein ID登记号】
NP_033408
UniProt accession no.【UniProt登记号】
P01831
shipped in【运输】
ambient
storage temp.【储存温度】
2-8℃
Gene Information
mouse ... Thy1(21838)
compatibility【相容性】
Requires ColorWheel® Dye (Sold Separately)
基本信息
General description【一般描述】
ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
Specificity【特异性】
Clone 30-H12 is a rat monoclonal antibody that detects murine CD90.2 (Thy-1.2).
Immunogen【免疫原】
Mouse Thymus Cells.
Application【应用】
Quality Control Testing:

Evaluated by Flow Cytometry in Mouse splenocytes.

Flow Cytometry Analysis (FC): Staining of one million Mouse splenocytes was performed using 5 μL of 1:1 mixture of Cat. No. CWA-1008, Anti-Mouse CD90.2 (30-H12) ColorWheel® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye an equivalent amount of a PE-conjugated Rat IgG2b isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form【外形】
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution【重悬】
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Legal Information【法律信息】
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
产品说明
Target description【目标描述】
Thy-1 membrane glycoprotein (UniProt: P01831; also known as Thy-1 antigen, CD90) is encoded by the Thy1 (also known as Thy-1) gene (Gene ID: 21838) in murine species. CD90 is a highly conserved, GPI-anchored membrane protein with one Ig V-type superfamily domain (aa 20-127). It is synthesized with a signal peptide (aa 1-19) and a propeptide (aa 132-162) that are subsequently cleaved off to generate the mature form (aa 20-131). It lacks an intracellular domain but is located in the outer leaflet of lipid rafts at the cell plasma membrane allowing signaling functions by cis- and trans-interactions with G inhibitory proteins, the Src and c-fyn kinases, and tubulin. CD90 is a major cell surface protein found on both T-cells and in the brain. It plays a key role in cell-cell and cell-ligand interactions during synaptogenesis and other events in the brain. It also plays a role in the regulation of axon growth and nerve regeneration, T cell activation and apoptosis, leukocytes and melanoma cell adhesion and migration, and fibroblast proliferation and migration in wound healing. CD90 mRNA and protein expression has been reported in several cancer types including liver, myeloid, skin, and brain. It is considered as a marker for glioblastoma stem cells. Reduced expression of Thy-1 is shown to enhance the expression of osteogenic and adipogenic differentiation of mesenchymal stem cells. Clone 30-H12 does not cross react with mouse and rat strains bearing the Thy-1.1 alloantigen (Ref.: Sauzay, C., et al. (2019). Front. Cell Dev. Biol. 7; 66; Moraes, DA., et al. (2016). Stem Cell Res. Ther. 7; Article 97).
Storage and Stability【储存及稳定性】
Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 3
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