IkBa Protein, activated, 10 μg

32160405

NA.41

N-terminal GST-tagged, recombinant human Smurf1 full length, expressed in E coli.
IκBα is a cellular protein that inhibits the transcription factor NF-κB. NF-κB plays a central role in cellular stress and inflammatory responses by controlling cytokine-inducible gene expression and lymphocyte stimulation by antigens. In non-stimulated cells NF-κB is sequestered in the cytoplasm via the inhibitory protein IκBα, which obscures the nuclear localization signal of NF-κB and also blocks its ability to bind DNA. In response to various stimuli, including viral infection, ultraviolet radiation and inflammatory cytokines, IκBα is phosphorylated by the protein kinase complex IKK, which triggers its rapid degradation by ubiquitin mediated proteolysis. The liberated NF-κB then translocates to the nucleus and activates the expression of target genes. Mutations in the gene for IκBα result in the functional impairment of NF-κB which causes a decreased production of proinflammatory cytokines and certain interferons, rendering patients susceptible to infection.

Ubiquitination Cascade Comoponent: Substrate

Purified using immobilized metal affinity chromatography.

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

300
100

human

Upstate^®

activity assay: suitable

M69043

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12 - Non Combustible Liquids

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