DAF-2-CAS 205391-01-1-Calbiochem

C₂₀H₁₄N₂O₅

362.34

MFCD05865100

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T, Cat. No. 251510). Can detect NO under neutral conditions (detection limit = 5 nM).
A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.

Primary Target
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

Toxicity: Irritant (B)

A 5 mM (1 mg in 550 µl) solution in DMSO.

Following initial thaw, aliquot and freeze (-20°C).

Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (HPLC)

solution

Calbiochem^®

OK to freeze
protect from light

pale yellow

λ_ex ~495 nm
λ_em ~515 nm

−20℃

10 - Combustible liquids

WGK 2

188.6 °F - (Dimethylsulfoxide)

87 ℃ - (Dimethylsulfoxide)