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Goat Anti-Rabbit IgG Peroxidase Conjugate
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | goat |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | secondary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | lyophilized |
does not contain【不包含】 | preservative |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | 200 μg in Plastic ampoule |
基本信息
General description【一般描述】 | This Goat Anti-Rabbit IgG Peroxidase Conjugate is validated for use in ELISA, Immunohistochemistry for the detection of Rabbit IgG. Immunoaffinity purified goat polyclonal antibody adsorbed against human serum proteins and conjugated to horseradish peroxidase. Recognizes rabbit IgG. |
Application【应用】 | ELISA (1:5000-1:100,000) Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence) Immunohistochemistry (1:500-1:5000) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | Lyophilized from 250 mM NaCl, 10 mM sodium phosphate, 15 mg/ml BSA, pH 7.6. |
Reconstitution【重悬】 | Reconstitute the antibody with 1.5 ml H₂O. Dissolve by gently mixing at 4°C, since extensive mixing will denature antibodies. Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Alternatively, glycerol (to a final concentration of 50%) may be added and the reconstituted antibody then stored at -20°C. Please note, under these conditions the concentration of the antibody and the buffer salts will be one-half of the original concentration. |
Other Notes【其他说明】 | To ensure the quality of these reagents each lot is tested with several of our primary antibodies. Antibody specificity is tested by immunoelectrophoresis and shown to be specific for rabbit IgG and light chains common to most rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins has been detected. The antibody has also been tested by ELISA and/or adsorbed to ensure minimal cross-reactivity with human serum proteins, but the antibody may exhibit cross-reactivity with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 3 |