您的位置：首页 > 产品中心 > Anti-mSin1 Antibody
Anti-mSin1 Antibody
| 产品编号： | 4094007 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-mSin1 Antibody
Target of rapamycin complex 2 subunit MAPKAP1, TORC2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, Stress-activated map kinase-interacting protein 1, SAPK-interacting protein 1, mSIN1, mSin1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Target of rapamycin complex 2 subunit MAPKAP1 (UniProt Q9BPZ7; also known as MEKK2-interacting protein 1, Mitogen-activated protein kinase 2-associated protein 1, mSIN1, Ras inhibitor MGC2745, SAPK-interacting protein 1, Stress-activated protein kinase-interacting 1, Stress-activated map kinase-interacting protein 1,, TORC2 subunit MAPKAP1) is encoded by the MAPKAP1 (also known as JC310, MIP1 SIN1, SIN1b, SIN1g) gene (Gene ID 79109) in human. mSIN1 is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase, along with mSIN1, GbL (mLST8), Protor1, Protor2, and Rictor. The mTORC2 complex is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is known to affect cytoskeletal organization and cell migration by exerting its effects through Rac, Rho, and PKC. mSIN1 is also known to directly interact with Ras, SPAKs, and JNK. |
| Specificity【特异性】 | Expected to react with spliced isoforms 1, 2, 3, 5, 6, but not 4. |
| Immunogen【免疫原】 | Epitope: MAP3K2/NBN interaction domain KLH-conjugated linear peptide corresponding to the MAP3K2/NBN interaction domain of human mSin1. |
| Application【应用】 | Research Category Signaling Research Sub Category Signaling Neuroscience Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated mSin1 in 0.5 mg of A431 cell lysate. Detect MAPKAP1/Sin1 using this rabbit polyclonal Anti-mSin1 Antibody, Cat. No. 07-2276-I, validated for use in Western Blotting and Immunoprecipitation. |
| Quality【质量】 | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected mSin1 in 10 µg of A431 cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | rat, mouse, human |
| species reactivity (predicted by homology) | hamster (based on 100% sequence homology), porcine (based on 100% sequence homology), bovine (based on 100% sequence homology), feline (based on 100% sequence homology), horse (based on 100% sequence homology) |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_001006618 |
| UniProt accession no.【UniProt登记号】 | Q9BPZ7 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... MAPKAP1(79109) |
产品说明
| Target description【目标描述】 | ~59 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
