Anti-Caspase 1 Antibody

32160702

NA.41

Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an enzyme that cleaves the inflammatory cytokines IL-1 beta and IL-18, releasing the mature, active peptides. Caspase-1 is important for defense against pathogens and it also is involved in the induction of apoptosis. Thymocytes from caspase-1 deficient mice are resistant to Fas-mediated apoptosis, and caspase-1 is similar to the cell death gene CED-3 of C. elegans. Caspase-1 is a heterotetramer that consists of two anti-parallel heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The enzyme has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD). Caspase-1 is highly expressed in spleen and lung, and is absent from brain.

This antibody recognizes the p20 subunit of Caspase 1. This antibody may cross-react with Caspase 5 due to 92% sequence homology. Additionally, this antibody recognizes the P45 proenzyme (~45 kDa) and the p20 subunit (~20 kDa) of the reduced, human Caspase 1 isoforms.
Other homologies: Mouse, rat, canine, porcine and feline. (92% sequence homology).

KLH-conjugated linear peptide corresponding to the p20 subunit of human Caspase 1.
Epitope: p20 subunit

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Caspase 1 in human spleen tissue. Additionally, a 1:4,000 dilution from a representative lot detected Caspase 1 in human colon tissue.
Anti-Caspase 1 Antibody is a highly specific rabbit polyclonal antibody, that targets Caspase 1 & has been tested in western blotting & IHC (Paraffin).
Research Sub Category
Caspases

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 5 µg/mL of this antibody detected Caspase 1 in 10 µg THP-1 cell lysate.

Replaces: 06-503

Affinity purified

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

immunohistochemistry: suitable (paraffin)
western blot: suitable

NP_001214

P29466

wet ice

~45 kDa observed. Five isoforms between 10-45 kDa are known to exist. This antibody recognizes the P45 proenzyme (~45 kDa) and the p20 subunit (~20 kDa) of the reduced, human Caspase 1 isoforms.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1