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Anti-PNMT Antibody
| 产品编号： | 4093927 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-PNMT Antibody
Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT, PEMT2
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | PNMT, also known as Phosphatidylethanolamine N-methyltransferase, or PEMT, PEAMT, or PEMT2, and encoded by the gene PEMT/PEMPT/PNMT, catalyzes three sequential methylation reactions of phosphatidylethanolamine to produce phosphatidylcholine a major constituent of cell membranes and a primary pulmonary surfactant. PNMT/PEMT is localized to the endoplasmic reticulum membrane as well as the mitochondrial membranes. PNMT/PEMT is widely expressed, and has the highest activity in the mitochondria. The conversion of phosphatidylethanolamine to phosphatidylcholine by sequential methylation via PNMT/PEMT occurs in the liver. A different pathway for phosphatidylcholine production starting from choline also is present in cells but it does not involve PNMT/PEMT enzyme. Mutations in PNMT/PEMT protein are associated with nonalcoholic fatty liver disease (NAFLD). |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to the C-terminus of human PNMT. Epitope: C-terminus |
| Application【应用】 | Immunohistochemistry Analysis: A 1:500 dilution and a 1:2,000 dilution from a representative lot detected PNMT in human adrenal gland and testis tissues, respectively. Research Sub Category Signaling Neuroscience Research Category Neuroscience Anti-PNMT Antibody detects level of PNMT & has been published & validated for use in PNMT. |
| Quality【质量】 | Evaluated by Western Blotting in human liver tissue lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected PNMT in 10 µg of human liver tissue lysate. |
| Linkage【联系】 | Replaces: AB110 |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_001254480 |
| UniProt accession no.【UniProt登记号】 | Q9UBM1 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~17 kDa observed. As isoform of PNMT has been observed between 15-20 kDa (Cui, Z., et al. (1993). J Biol. Chem. 268(22):16655-16663.). |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
