Goat Anti-Human IgG Antibody, biotin conjugate

32160702

NA.46

IgG is a monomeric immunoglobulin, consists of two heavy chains (gamma chains) and two light chains. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in interstitial fluid, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, providing protection to the fetus in its first weeks of life before its own immune system has developed. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

The antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.

Prepared from purified human IgG.

Research Category
Secondary & Control Antibodies
EIA: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Western blots: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Immunohistochemistry: 1:500-1:5,000 using enzyme-streptavidin conjugate.
Flow cytometry: 1:200-1:1,000.
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
This Goat anti-Human IgG Antibody, biotin conjugate is validated for use in ELISA, WB for the detection of Human IgG.

Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.
ImmunoAffinity Purified

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

goat

biotin conjugate

affinity purified immunoglobulin

secondary antibodies

polyclonal

human

Chemicon^®

ELISA: suitable
western blot: suitable

wet ice

Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS07

Warning

H312 + H332 - H412

P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

11 - Combustible Solids

WGK 3