Anti-α-Tubulin Mouse mAb (DM1A)

100

mouse

purified antibody

primary antibodies

DM1A, monoclonal

liquid

≤0.1% sodium azide as preservative

human, gerbil, rat, chicken, mouse

Calbiochem^®

do not freeze

IgG1

wet ice

2-8℃

Please refer to vial label for lot-specific concentration.

This Anti-α-Tubulin Mouse mAb (DM1A) is validated for use in Immunoblotting, Immunofluorescence for the detection of α-Tubulin.
Purified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa tubulin protein.
Recognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.

Chicken
native chick brain microtubules

Immunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references)

Immunofluorescence (5 µg/ml)

Toxicity: Standard Handling (A)

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Positive Control
Any cell line
Negative Control
trp E (Ab-1)

Tubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.
Blose, S.H., et al. 1984. J. Cell. Biol.98, 847.
Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 1