Anti-Partitioning-defective 3 Antibody, Alexa Fluor^® 488 Conjugate

32160702

Partitioning defective 3 homolog (UniProt Q8TEW0; also known as ASIP, Atypical PKC isotype-specific-interacting protein, Bazooka, CTCL tumor antigen se2-5, Par-3 family cell polarity regulator alpha, Par-3 partitioning defective 3 homolog, PAR3-alpha, Protein phosphatase 1 regulatory subunit 118) is encoded by the PARD3 (also known as Baz, PAR3, PAR3A, PARD3A, PPP1R118) gene (Gene ID 56288) in human. Par3 is a PDZ (PSD95/Dlg/ZO-1) domain-containing membrane scaffold adapter protein that plays an important role in epithelial cell tight junction (TJ) formation and apico-basal polarity maintenance. Together with PAR6, atypical protein kinase C (aPKC), and the Rho GTPase CDC42, Par3 forms a complex that regulates cytoskeleton re-organization, post-golgi vesicle trafficking, microtubule network polarization, and asymmetric cell division. PAR6 interacts directly with aPKC isoforms iota/lambda and zeta to form the complex core. Recruitment of GTP-bound CDC42 to the PAR6-aPKC core induces aPKC phosphorylation/activation by PAR6, and subsequent PAR3 binding. Another quaternary apical junctional complex (AJC), Willin–Par6-aPKC–Par3, has also been reported. In this case, the complex-associated aPKC functions to phosphorylate ROCK, resulting in ROCK dissociation from the AJC and thereby preventing excessive ROCK-dependent apical constriction.

Expected to react with all 11 spliced isofoms (UniProt Q8TEW0-1 to Q8TEW0-11).

GST fusion protein corresponding to residues 457-744 of mouse PAR-3 (accession number AY02657).

Research Category
Cell Structure
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Partitioning-defective 3 in A431, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-330) is shown to be suitable also for Western blotting, immunocytochemistry, and immunohistochemistry applications.
This Anti-Partitioning-defective 3 Antibody, Alexa Fluor^® 488 Conjugate is validated for use in Immunocytochemistry for the detection of Partitioning-defective 3.
Research Sub Category
Adhesion (CAMs)

Evaluated by Immunocytochemistry in HUVECs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Partitioning-defective 3 in HUVECs.

Purified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Protein A purified

Concentration: Please refer to lot specific datasheet.

ALEXA FLUOR is a registered trademark of Life Technologies

rabbit

100

ALEXA FLUOR^® 488

purified antibody

primary antibodies

polyclonal

mouse, rat, monkey, frog, canine, human

immunocytochemistry: suitable

NP_062565

Q8TEW0

wet ice

human ... PARD3(56288)

~180, 150, 100 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

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12 - Non Combustible Liquids

WGK 2