您的位置：首页 > 产品中心 > Anti-Sp1 Antibody
产品别名
Anti-Sp1 Antibody
Sp1 transcription factor, specificity protein 1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Sp1 was one of the first transcription factors to be characterized in mammalian cells. The Sp1 protein plays a role in the regulation of gene expression. This protein has three Cys2His2 zinc-finger motifs that have been shown to bind to the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ also known as a GC box. Sp1 is a ubiquitous transcription factor required for the expression of a variety of genes, such as those involved in the cell cycle and development. Interaction of the Sp1 protein with the GC box activates transcription of specific mRNAs from genes that contain functional recognition sites. |
| Specificity【特异性】 | This antibody recognizes Sp1. Homology to chimpanzee, canine, and rhesus monkey is 99%. |
| Immunogen【免疫原】 | GST-tagged recombinant protein corresponding to full-length human Sp1. |
| Application【应用】 | Anti-Sp1 Antibody is a Rabbit Polyclonal Antibody for detection of Sp1 also known as Sp1 transcription factor, specificity protein 1 & has been validated in WB, IP, ICC. Research Sub Category Transcription Factors Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Sp1 in HeLa and A431 cells. Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract. Research Category Epigenetics & Nuclear Function |
| Quality【质量】 | Evaluated by Western Blot in HeLa nuclear extract. Western Blot Analysis: 0.1 µg/mL of this antibody detected Sp1 on 10 µg of HeLa nuclear extract. |
| Linkage【联系】 | Replaces: MABE550 |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. Format: Purified Protein A purified |
| Analysis Note【分析说明】 | Control HeLa nuclear extract |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| species reactivity | mouse, human, rat |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_612482 |
| UniProt accession no.【UniProt登记号】 | P08047 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | 95 kDa and 105 kDa |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
