PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

liquid

preservative

mouse, human, rat, monkey

Calbiochem^®

OK to freeze

IgG

wet ice

−20℃

50 μL in Plastic ampoule

This PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Chk1 (pSer³¹⁷).
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser³¹⁷.
Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser³¹⁷ in UV-treated COS cells.

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1

Immunoblotting (1:1000)
Immunocytochemistry (1:500, fluorescence)
Paraffin Sections (1:50)

Toxicity: Standard Handling (A)

In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Do not aliquot.

Positive Control
Lysates from COS cells treated with UV

Does not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol.21, 4129.
Shieh, S.Y., et al. 2000. Genes Dev.14, 289.
Martinho, R.G., et al. 1998. EMBO J.17, 7239.
Zeng, Y., et al. 1998. Nature395, 507.

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10 - Combustible liquids

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