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产品别名
C99
C-99
C 99
产品性质
| biological source【生物来源】 | human colon |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Epithelial |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
基本信息
| Cell Line Origin【细胞系来源】 | Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage C |
| Cell Line Description【细胞系描述】 | The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes′ stage C. |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 8,14 D16S539: 11,13 D5S818: 11,12 D7S820: 9,11 THO1: 8,9.3 TPOX: 8,10 vWA: 16,17 |
| Culture Medium【培养基】 | Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 ℃. C99 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
产品说明
| Disclaimer【免责声明】 | This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form. |
