InSolution Ginkgolic Acid (15:1)-CAS 22910-60-7-Calbiochem

C₂₂H₃₄O₃

346.50

MFCD03093717

A cell-permeable anacardic acid (Cat. No. 172050) analog that inhibits protein SUMO (Cat. No. 662037, 662039, and 662042) modification (IC₅₀ = 3.0 µM using RanGAP1-C2 as substrate) in an ATP-dependent manner by selectively targeting SUMO-activating enzyme E1 (Cat. No. 662073 and 662074) and interfering with E1-SUMO intermediate formation independent of E1 active site cysteine. Both ginkgolic acid and anacardic acid are shown to effectively decrease overall SUMOylation of 293T cellular proteins, including p53, in a dose-dependent manner, while neither compound is effective in affecting overall cellular protein ubiquitination or histone H4K8 acetylation in 293T cultures, although both compounds are shown to inhibit histone lysine acetylation by PCAF (Cat. No. 124026) in cell-free acetylase assays.

Cell permeable: yes
Primary Target
SUMO-activating enzyme E1
Reversible: yes

Toxicity: Standard Handling (A)

A 25 mM (2 mg/231 µl) sterile-filtered solution of Ginkgolic Acid (15:1) (Cat. No. 345887) in DMSO.

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C. Use only fresh DMSO for further dilutions.

Fukuda, I., et al. 2009. Chem. Biol.16, 133.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (HPLC)

liquid

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles
desiccated (hygroscopic)
protect from light

−70℃

1S/C22H34O3/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-16-19-17-15-18-20(23)21(19)22(24)25/h7-8,15,17-18,23H,2-6,9-14,16H2,1H3,(H,24,25)/b8-7-

YXHVCZZLWZYHSA-FPLPWBNLSA-N

Packaged under inert gas

10 - Combustible liquids

WGK 2

188.6 °F - (refers to pure substance)

87 ℃ - (refers to pure substance)