InSolution Aphidicolin-CAS 38966-21-1-Calbiochem

C₂₀H₃₄O₄

338.48

MFCD00083214

A cell-permeable tetracyclic diterpene antibiotic. Cell synchronization agent. Blocks the cell cycle at the early S-phase. Specific, non-competitive and reversible inhibitor of DNA polymerase α and δ in eukaryotic cells and in some viruses of animal origin. Potentiates apoptosis induced by arabinosyl nucleosides in leukemia cell lines. Also induces apoptosis in HeLaS3 cells, but inhibits vincristine (>Cat. No. 677181)-induced apoptosis in the p53-negative human prostate cancer cell line PC-3.

Cell permeable: yes
Primary Target
DNA polymerase α and δ
Reversible: yes

Toxicity: Irritant (B)

A 30 mM (1 mg/98 µL) sterile-filtered solution of Aphidicolin (Cat. No. 178273) in DMSO.

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C. Use only fresh DMSO for further dilutions.

Borner, M.M., et al. 1995. Cancer Res.55, 2122.
Poluha, W., et al. 1995. Oncogene10, 185.
Urbani, L., et al. 1995. Exp. Cell Res.219, 159.Schimke, R.T., et al. 1994. Philos. Trans. R. Soc. London. B. Biol. Sci.345, 311.Kuwakado, K., et al. 1993. Biochem. Pharmacol.46, 1909.Hubermann, J.A. 1981. Cell23, 647.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

≥98% (TLC)

100

liquid

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles
desiccated (hygroscopic)
protect from light

−70℃

1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1

NOFOAYPPHIUXJR-APNQCZIXSA-N

Packaged under inert gas

10 - Combustible liquids

WGK 2

188.6 °F - (refers to pure substance)

87 ℃ - (refers to pure substance)