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Anti-Caspase-8 Antibody, clone 1L15 ZooMAb® Rabbit Monoclonal

产品编号:4083007
规格:recombinant, expressed in HEK 293 cells
包装规格:25 μL,4 X 25 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
408300725 μL2350
40830074 X 25 μL4400
产品性质
Quality Level【质量水平】
200
biological source【生物来源】
rabbit
recombinant【重组】
expressed in HEK 293 cells
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
1L15, recombinant monoclonal
description【描述】
recombinant, expressed in HEK 293 cells
product line【产品线】
ZooMAb® learn more
form【形式】
lyophilized
mol wt【分子量】
calculated mol wt 55.39 kDa
observed mol wt ~55 kDa
species reactivity
human
packaging【包装】
antibody small pack of 25 μL
enhanced validation【增强验证】
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype【同位素/亚型】
IgG
epitope sequence【表位序列】
C-terminal half
Protein ID accession no.【Protein ID登记号】
NP_001073595
UniProt accession no.【UniProt登记号】
Q14790
shipped in【运输】
ambient
storage temp.【储存温度】
2-8℃
基本信息
General description【一般描述】
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.
Specificity【特异性】
Clone 1L15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Caspase-8. It targets an epitope within the p18 subunit
Immunogen【免疫原】
KLH-conjugated linear peptide corresponding to 15 amino acids from the p18 subunit of human Caspase-8.
Application【应用】
Anti-Caspase-8, clone 1L15 ZooMAb®, Cat. No. ZRB1426, is a recombinant Rabbit monoclonal antibody that detects Caspase-8 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Caspase-8 in HeLa cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Caspase-8 in lysate from Jurkat cells treated with Staurosporin.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Caspase-8 in HUVEC and A431 cells.

Affinity Binding Assay: A representative lot of this antibody bound Caspase-8 with a KD of 1 x 10-12 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Caspase-8 in human colon and kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form【外形】
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution【重悬】
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Legal Information【法律信息】
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
产品说明
Target description【目标描述】
Caspase-8 (UniProt: Q14790; also known as EC:3.4.22.61, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, MACH) is encoded by the CASP8 (also known as MCH5) gene (Gene ID: 841) in human. Caspase-8 is a cysteine protease that plays a major role in the extrinsic apoptotic signaling pathway via death receptors. In its inactive form, it associates with FADD by interactions between their DED motifs and within the FAS-FADD-Caspase 8 complex caspase 8 undergoes self-cleavage to convert to the active form. Activated caspase 8 is subsequently released to the cytosol and it cleaves downstream effector caspases 3, 4, 6, 7, 9 and 10. Highest expression of caspase-8 is found in peripheral blood leukocytes, spleen, thymus, and liver and it is barely detectable in brain, testis, and skeletal muscle. Caspase-8 is produced with two propeptides (aa 1-216 and 375-384) that are cleaved under appropriate signals to produce a p18 (aa 217-374) and a p10 (aa 385-479) subunit. Active caspase 8 is a heterotetramer that consists of two p18 and two p10 subunits. In human, nine different isoforms of caspase-8 have been described that are produced by alternative splicing. Isoforms 5, 6, 7, and 8 lack the e catalytic site and may interfere with the pro-apoptotic activity of the complex. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Storage and Stability【储存及稳定性】
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 1
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