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Anti-Src Antibody, CT, clone NL19 | 04-772
产品别名
Anti-Src Antibody, CT, clone NL19 | 04-772
Proto-oncogene tyrosine-protein kinase SRC, Protooncogene SRC, Rous sarcoma tyrosine kinase pp60c-src, Tyrosine-protein kinase SRC-1, v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain. |
| Specificity【特异性】 | Predicted to cross react with frog and chicken. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck. Src-tyrosine kinase family |
| Immunogen【免疫原】 | KLH conjugated synthetic peptide (CKVADFGLARLIEDNEYTARQGA) of Src (avian sarcoma virus), residues 400-422. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck. |
| Application【应用】 | Western Blot Analysis: A 1:2000 to 1:8000 dilution of this lot detected Src in RIPA whole cell lysates from human A431 cells. Immunoprecipitation: 5-20 μL of a previous lot immunoprecipitated Src from 500 μg of A431 RIPA lysate. |
| Quality【质量】 | routinely evaluated by immunoblot on RIPA whole cell lysates from human A431 and Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells |
| Linkage【联系】 | Replaces: 05-772 |
| Physical form【外形】 | Format: Purified |
| Analysis Note【分析说明】 | Control RIPA whole cell lysates from human A431, Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells Included Positive Antigen Control: Catalog # 12-301, Non-Stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. |
| Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | culture supernatant |
| antibody product type | primary antibodies |
| clone【克隆】 | NL19, monoclonal |
| species reactivity | mouse, rat, human |
| manufacturer/tradename | Upstate® |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG |
| NCBI accession no.【NCBI登记号】 | NM_005417 |
| UniProt accession no.【UniProt登记号】 | P12931 |
| shipped in【运输】 | dry ice |
| Gene Information | human ... SRC(6714) |
产品说明
| Target description【目标描述】 | ~53-62kDa |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
