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Anti-PICH Antibody, clone 142-26-3
产品别名
Anti-PICH Antibody, clone 142-26-3
excision repair cross-complementing rodent repair deficiency, complementation group 6-like, excision repair cross-complementing rodent repair deficiency complementation group 6-like, Tumor antigen BJ-HCC-15, PLK1-interacting checkpoint helicase, ATP-de
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Plk1-interacting checkpoint helicase (PICH ) is an essential component of the spindle assembly checkpoint (Baumann, C., et al. (2007). Cell 128:101–114). This protein is an ATPase family SNF2 member, and serves as a binding partner and substrate for Plk1. When PICH is phosphorylated, it retrieves Plk1 which serves to mediate the localization of PICH. As a DNA helicase, PICH gathers at kinetochores and centromeres during prometaphase, acting as a necessary part of the spindle assembly checkpoint by recruiting MAD2 and mediating chromatin centromeric tension. Depleted PICH results in the cancellation of the spindle checkpoint causing large scale missegregation of the chromosome. |
| Specificity【特异性】 | This antibody recognizes Plk1-interacting checkpoint helicase. |
| Immunogen【免疫原】 | Histidine-tagged recombinant protein corresponding to human Plk1-interacting checkpoint helicase. Epitope: Unknown |
| Application【应用】 | Research Sub Category Cell Cycle, DNA Replication & Repair Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. Research Category Epigenetics & Nuclear Function Use Anti-PICCH Antibody, clone 142-26-3 (mouse monoclonal antibody) validated in WB, IP, ICC to detect PICCH also known as Tumor antigen BJ-HCC-15, PLK1-interacting checkpoint helicase, ATP-dependent helicase ERCC6-like. |
| Quality【质量】 | Evaluated by Western Blot in HeLa-S3 cell lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected Plk1-interacting checkpoint helicase on 10 µg of HeLa-S3 cell lysate. |
| Physical form【外形】 | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. Format: Purified Protein G Purified |
| Analysis Note【分析说明】 | Control HeLa-S3 cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 142-26-3, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG1κ |
| NCBI accession no.【NCBI登记号】 | NP_060139 |
| UniProt accession no.【UniProt登记号】 | Q2NKX8 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... ERCC6L(54821) |
产品说明
| Target description【目标描述】 | ~160 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
