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Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate
产品编号: | 4080005 |
规格: | suspension (Liquid), clone Y13-259, Calbiochem® |
包装规格: | .5 ML |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | rat |
antibody product type | primary antibodies |
clone【克隆】 | Y13-259, monoclonal |
form【形式】 | suspension (Liquid) |
contains【包含】 | ≤0.1% sodium azide as preservative |
species reactivity | rat, mouse, human |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | do not freeze |
isotype【同位素/亚型】 | IgG1 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | .5 ml in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
General description【一般描述】 | Recognizes the ~21 kDa ras protein in SW480 and Y1 cells and normal skin tissue. For neutralization studies, Cat. No. OP01L is recommended. For immunoprecipitation, Cat. No. OP01A is recommended. This Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate is validated for use in Immunoprecipitation, Other Applications for the detection of v-H-Ras (Ab-1). Rat monoclonal antibody generated by immunizing Sprague Dawlay rats with the specified immunogen and fusing splenocytes with Y3Ag1.2.3 rat myeloma cells. Conjugated to agarose. Recognizes the ~21 kDa ras protein (see comments). |
Immunogen【免疫原】 | recombinant p21 Ras |
Application【应用】 | Immunoprecipitation (15 µl beads or 1 µg antibody per sample, see comments) Other Applications (see comments) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In PBS. |
Analysis Note【分析说明】 | Positive Control SW 480 or Y1 cells or normal skin tissue |
Other Notes【其他说明】 | Agarose conjugated recommended for immunoprecipitation. For frozen sections, paraffin sections, immunoblotting, neutralization, and immunofluoresence use Cat. No. OP01 or OP01L. Broadly reactive by immunoprecipitation with both phosphorylated and non-phosphorylated forms of v-H-ras and v-K-ras (see application references) and reacts with the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl agarose conjugate is recommended to be used as an immunoconcentrator prior to western blot analysis of p21ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems. Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112. Taparowsky, E., et al. 1983. Cell34, 581. Ellis, R.W., et al. 1981. Nature292, 506. Shih, T.Y., et al. 1980. Nature287, 686. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |