Anti-Green Fluorescent Protein Antibody

32160702

NA.41

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Reacts with Green Fluorescent Protein (GFP). The antibody reacts with various forms of GFP including eGFP including those found in vectors supplied by Clonetech and Invitrogen. There is not cross-reactivity to RFP. YFP has not been tested. This antibody works well for immunohistochemistry.

GFP-BSA

Western blot: 1:2,500-1:5,000. Recommended dilution buffer is PBS containing 1 mg/mL BSA.

Immunocytochemistry: 1:500-1:1,000

Immunohistochemistry: 1:500-1:1,000 on paraformaldehyde fixed tissues with an overnight incubation using Nickel enhanced DAB for detection. Suggested permeabilization method is 0.5% Triton X-100. Recommended blocking buffer is PBS containing BSA and serum from the host of the secondary antibody. Recommended dilution buffer is PBS containing 1 mg/mL of BSA.

ELISA: 1:1,000-1:2,000 in a direct assay.

Optimal working dilutions must be determined by the end user.
Detect Green Fluorescent Protein using this Anti-Green Fluorescent Protein Antibody validated for use in ELISA, IC, IH & WB.

Protein A purified
Liquid in PBS containing 1 mg/mL BSA. Contains no preservative.
Format: Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

affinity purified immunoglobulin

primary antibodies

monoclonal

all

Chemicon^®

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

IgG1

P42212

MW for GFP is 27 kDa Dependent upon the molecular weight of the fusion protein being detected.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2