单克隆抗-β-连环蛋白

MFCD00282178

NA.41

Anti-β-Catenin-Cy3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant chicken catenin. β-catenin is 92 kDa. β-catenin protein is composed of a series of protein-protein interaction motifs that allow it to function as a scaffold. The N-terminus domain contains the binding site for β-catenin as well as phosphorylation sites recognized by Glycogen synthase kinase 3 beta (GSK3β) and the C-terminus contains the transcriptional activation domain and binding site for Teashirt.
The gene β-catenin (CTNNB1) is mapped to human chromosome 3p21. The protein localizes to the cytoplasm and the nucleus. β-catenin is also present at the centrosomes.

特异性识别哺乳动物和禽类β-连环蛋白。不与斑珠蛋白(γ-连环蛋白)发生交叉反应。

重组鸡β-连环蛋白。

Suitable for direct immunofluorescence at a dilution of 1:100 using methanol/acetone-fixed bovine MDBK/MCF-7 cells. The antiobody has been used for western blot analysis in mouse embryonic stem cells.
Anti-β-Catenin-Cy3 antibody has been used in

• direct immunofluorescence
• western blotting
• staining
• immunohistochemistry

Catenin (cadherin-associated protein) β 1 also called β-catenin is encoded by the CTNNB1 gene in humans. It is a dual function protein that regulates the coordination of cell-cell adhesion and gene transcription. It is useful for differential diagnosis of osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and provide a potential target for CCA therapy. Mutations in β-catenin are associated with ovarian carcinoma. In addition, mutations in β-catenin are linked to intellectual disability. NEK2 (Never in mitosis A-related kinase 2) binds and phosphorylates β-catenin, thereby regulating centrosome seperation. Focal adhesion protein, Kindlin-2, enhances WNT signaling by forming a transcriptional complex with β-catenin.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Cy is distributed under license from Amersham Biosciences Limited.

200

mouse

CY3 conjugate

purified immunoglobulin

primary antibodies

15B8, monoclonal

buffered aqueous solution

antigen 94 kDa

human, bovine, chicken, canine

~1 mg/mL

direct immunofluorescence: 1:100 using methanol/acetone-fixed bovine MDBK/MCF-7 Cell Line

IgG1

P35222

wet ice

2-8℃

human ... CTNNB1(1499)

200 μL in glass insert

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12 - Non Combustible Liquids

WGK 2