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广州伟伯科技有限公司

广州伟伯科技有限公司

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产品中心

MS β-Glucuronidase

产品编号：

4079357

规格：	Prebuffered, automation-ready hydrolysis mix

包装规格：

5 ML，10 ML，50 ML，100 ML

产品类别：

进口试剂

品牌：	Sigma-Aldrich

优惠价：

产品价格

产品编号	包装单位	单价(元)	国内现货	国外库存	询价单
4079357	5 ML	1230
4079357	10 ML	2830
4079357	50 ML	11280
4079357	100 ML	16850
4079357	Prebuffered, automation-ready hydrolysis mix	35430
4079357	Prebuffered, automation-ready hydrolysis mix	66620
4079357	Prebuffered, automation-ready hydrolysis mix	123920

产品别名

MS β-Glucuronidase

beta-Gluc

GUS

MS beta-Glu

bgluc

产品性质

Quality Level【质量水平】	200
shipped in【运输】	ambient
storage temp.【储存温度】	2-8℃

基本信息

General description【一般描述】	Protocol: MS β-Glucuronidase Quick Start Guide MS β-Glucuronidase is a stable recombinant β-Glucuronidase mix for robust urine drug testing. Hydrolysis is completed at room-temperature or in your autosampler in a single-step . The stability and low-temperature compatibility allows an analyst to use MS β-Gluc as a bench-top reagent. Reliable hydrolysis over days on the bench means less hands-on time, less frequent reagent prep, and more automation. It has been verified against a broad spectrum of conjugated analytes, including “hard to cleave” glucuronides, particularly opiates and opioids. MS β-Gluc eliminates sample preparation bottlenecks while enabling fully automated hydrolysis and reproducible analytical results.
Application【应用】	Urine sample preparation in Forensic and Clinical toxicology laboratories Enzymatic hydrolysis of drug glucuronide conjugates for urine drug testing by LC/MS Forensic drug testing, workplace and prescription drug monitoring, antidoping, SAMHSA Compatible with wide range of glucuronide drug conjugates, including but not limited to opioids, opiates, benzodiazepines and cannabinoids and hormones.
Features and Benefits【特点和优势】	Opened bottle retains 97% activity at 25℃ after 2 months. Stable on the bench in MasterMix (with methanol): Retains 99% activity for a week. Pre-buffered for use with human urine with volatile buffer ammonium acetate for LC-MS. Single-step hydrolysis at room-temperature or within autosampler (20-40℃). Verified with real human urine samples, not synthetic. “Walk-away” workflow. Automation compatibility (oven and centrifuge not required). Purified recombinant enzyme
Preparation Note【制备说明】	For Opiates, Opioids and Broad-spectrum drug hydrolysis use 80 to 160 μL of MS ß-Glucuronidase per reaction for a 200 μL reaction in urine, so 6-12 samples can be hydrolyzed per mL of MS beta-Gluc depending on reaction temperature. Dilute-and-shoot workflows: add MS β-glucuronidase formula and internal standards in methanol to urine and immediately transfer to autosampler at 40℃. After 16min switch temperature to 4℃. Hydrolyzed samples are ready for injection. Workflows with extraction or filtration step: add MS β-glucuronidase formula and internal standards in methanol to urine. Incubate 30 min at room-temperature. Proceed with extraction or filtration.

产品说明

Storage and Stability【储存及稳定性】

Long term Storage: 24 months at 2 - 8 ℃.
Ambient for 2 months.
Ambient for 1 week in Master Mix (11% methanol).

安全信息

Storage Class Code【储存分类代码】	10 - Combustible liquids
WGK	WGK 3

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