DKB human cell line

human blood

Dutch

female

Suspension

Not specified

Lymphoblastoid

cell culture | mammalian: suitable

dry ice

Homozygous: Yes
Consanguineous: Yes

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

A: 2402
Cw: 304
B: 4001
HLA DRA: 101
HLA DRB1: 9012
HLA DRB4: 103
HLA DQAP: 3.2
HLA DQA1: 302
HLA DQB1: 3032
HLA DPA1: 1
HLA DPB1: 401

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 ℃. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

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HLA A: 24
HLA A ief: 24.1
HLA C: 10
HLA B: 60
HLA B ief: 60
Bw4/bw6: 6
HLA Dw: 23
HLA DR: 9
HLA DR53: 53
HLA DQ: 9
HLA DP: 4

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.