Anti-Mouse IgM (μ-chain specific)–Alkaline Phosphatase antibody produced in goat

MFCD00162657

NA.46

IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory. This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice.
Goat Anti-Mouse IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for mouse IgM when tested against purified mouse IgA, IgG and IgM myeloma proteins.
Binds mouse IgM; does not bind other mouse Igs.

Goat Anti-Mouse IgM (μ-chain specific)-Alkaline Phosphatase antibody has been used for ELISA and immunofluorescence assays. The antibody can also be used for IHC (1:50) and western blot (1:30,000) applications.
Mouse plasma antibody isotype determination was performed by ELISA using alkaline phopshatase-conjugated goat anti-mouse IgM as the secondary antibody.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Solution in 0.05 M Tris, pH 8.0, containing 1 mM MgCl₂, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

200

goat

alkaline phosphatase conjugate

affinity isolated antibody

secondary antibodies

polyclonal

buffered aqueous glycerol solution

mouse

direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

wet ice

2-8℃

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)