Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat

MFCD00162620

NA.46

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody detects mouse IgG (whole molecule). When tested by ELISA, the product reacts with the Fab fragment of mouse IgG but does not recognize the Fc fragment of mouse IgG, human IgG, IgA, IgM or rat IgG.

Purified mouse IgG

Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is suitable for use in western blot at 1:1000 dilution using human airway epithelial cells or using whole-cell extracts of rat tubal tissues . The antibody can also be used for immunohistochemistry (1:10 using formalin-fixed, paraffin-embedded sections).
Goat anti-mouse Fab specific alkaline phosphatase antibody was used to analyze serum Ig titers by His Sorb ELISA at 1:10000 at room temperature for two hours. Antibodies were diluted in PBS plus 0.2% BSA to reduce background.
Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.

Antibody adsorbed with human IgG and rat serum proteins.

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ with 50% glycerol as stabilizer and 15 mM sodium azide

Adsorbed to reduce background staining with human or rat samples.

200

goat

alkaline phosphatase conjugate

affinity isolated antibody

secondary antibodies

polyclonal

buffered aqueous solution

mouse

human, rat

direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10
western blot: 1:200,000-1:400.000 using assay detecting β-Actin in total cell extract of HeLa cells (5-10 μg per well)

wet ice

2-8℃

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 3

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)