抗-小鼠 IgG(全分子)-碱性磷酸酶 山羊抗

MFCD00162640

NA.46

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody react with normal mouse serum and mouse IgG using IEP. By ODD, the antiserum is reacts with mouse IgG1, IgG2a, IgG2b, IgG3, IgA and IgM. The conjugate shows no reactivity with human serum proteins by ELISA.

purified mouse IgG

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
在含有终浓度为0.5M NaCl的TBS/Tween中，使用碱性磷酸酶缀合的山羊抗小鼠IgG(μg/ ml)作为二抗，对支气管肺泡液中的表面活性蛋白A进行检测。
Human and Murine tumor cell lysate were analyzed for various protein expression by western blot using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary for 1 hour at 37 degrees.
Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody for immunocytochemistry assays and ELISA.

Antibody adsorbed with human serum proteins.

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 10 mM glycine, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide.

Adsorbed to reduce background with human samples.

200

goat

alkaline phosphatase conjugate

affinity isolated antibody

secondary antibodies

polyclonal

buffered aqueous glycerol solution

mouse

human

direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

wet ice

2-8℃

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12 - Non Combustible Liquids

WGK 2