Caspase-9 Substrate I, Fluorogenic-Calbiochem

C₃₃H₃₈F₃N₇O₁₁

765.69

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). May also act as a substrate for caspase-4 and caspase-5. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).

Product does not compete with ATP.
Primary Target
A fluorogenic substrate for caspase-9
Reversible: no
Cell permeable: no

Toxicity: Standard Handling (A)

Ac-Leu-Glu-His-Asp-AFC

Supplied as a trifluoroacetate salt.

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Ito, A., et al. 1999. Biochim. Biophys. Acta 1452, 263.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

solid

Calbiochem^®

OK to freeze
desiccated
protect from light

white

DMSO: 5 mg/mL

λ_ex ~400  nm
λ_em ~505 nm

ambient

−20℃

1 mg in Glass bottle
5 mg in Plastic ampoule

11 - Combustible Solids

WGK 1