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Citrate Assay Kit
产品编号: | 4071059 |
规格: | sufficient for 100 colorimetric or fluorometric tests |
包装规格: | 1 KIT |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品性质
usage【用途】 | sufficient for 100 colorimetric or fluorometric tests |
Detection Method | colorimetric fluorometric |
storage temp.【储存温度】 | −20℃ |
suitability【适用性】 | Suitable for citrate determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.) |
基本信息
General description【一般描述】 | Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays. |
Features and Benefits【特点和优势】 | Compatible with high-throughput handling systems. |
Principle【原理】 | In this kit, citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 3 |