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Human p53 ELISA Kit

产品编号:4067433
规格:for serum, plasma, cell culture supernatant and urine
包装规格:1 KIT
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40674331 KIT12870
基本信息
NACRES
NA.32
General description【一般描述】
Cellular tumor antigen p53 is a tumor-suppressor protein that comprises transactivation at N-terminus, central sequence-specific DNA-binding domain, and regulatory domain at C-terminus. It plays a key role in autophagy regulation. It inhibits tumors and acts as a cellular stress sensor especially during nutrient deprivation, hypoxia, and ribosome dysfunction. The Human p-53 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human p-53 in serum, plasma, cell culture supernatants and urine.
Immunogen【免疫原】
Recombinant Human p53
Application【应用】
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human p53 ELISA Kit has been used for quantifying p53 using enzyme-linked immunosorbent assay (ELISA) in human myeloma RPMI-8226 cells, human colorectal cancer HCT-116 cells, human breast cancer MCF-7 cells and human leukemiaHL-60 cells.
Other Notes【其他说明】
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Components【组分】
组份可单独销售
RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
RABELADD ELISA 5X Assay/Sample Diluent Buffer D (Item K)
RABSTOP3 ELISA Stop Solution (Item I)
RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
RABWASH4 20X Wash Buffer (Item B)
产品性质
species reactivity
human
packaging【包装】
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
capture ELISA: suitable
input
sample type urine
sample type serum
sample type plasma
sample type cell culture supernatant(s)
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.3 ng/mL
standard curve range: 0.328-80 ng/mL
Detection Method
colorimetric
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
Gene Information
human ... TP53(7157)
安全信息
Pictograms【象形图】
GHS05
Signal word【警示用语:】
Warning
Hazard Statements
H290
Precautionary Statements
P234 - P390
Hazard Classifications【危险分类】
Met. Corr. 1
Storage Class Code【储存分类代码】
8A - Combustible, corrosive hazardous materials
WGK
WGK 3
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